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Publications

 

2020

      Ripstein, Z. A.; Vahidi, S.; Rubinstein, J. L.; Kay, L. E. “A pH-Dependent Conformational Switch Controls N. Meningitidis ClpP Protease Function”. J. Am. Chem. Soc. 2020. https://doi.org/10.1021/jacs.0c09474.


      Conicella, A. E.; Huang, R.; Ripstein, Z. A.; Nguyen, A.; Wang, E.; Löhr, T.; Schuck, P.; Vendruscolo, M.; Rubinstein, J. L.; Kay, L. E. “An Intrinsically Disordered Motif Regulates the Interaction between the P47 Adaptor and the P97 AAA+ ATPase”. Proc. Natl. Acad. Sci. U. S. A. 2020, 117 (42), 26226–26236. https://doi.org/10.1073/pnas.2013920117.


      Guo, H.; Franken, E.; Deng, Y.; Benlekbir, S.; Lezcano, G. S.; Janssen, B.; Yu, L.; Ripstein, Z. A.; Tan, Y. Z.; Rubinstein, J. L. “Electron-Event Representation Data Enable Efficient CryoEM File Storage with Full Preservation of Spatial and Temporal Resolution”. IUCrJ 2020, 7 (5), 860–869. https://doi.org/10.1107/S205225252000929X.


      Huang, R.*; Ripstein, Z. A.*; Rubinstein, J. L.; Kay, L. E. “Probing Cooperativity of N-Terminal Domain Orientations in the P97 Molecular Machine: Synergy Between NMR Spectroscopy and Cryo-EM”. Angew. Chemie - Int. Ed. 2020. https://doi.org/10.1002/anie.202009767. *Co-first authorship.


      Vahidi, S.*; Ripstein, Z. A.*; Juravsky, J. B.; Rennella, E.; Goldberg, A. L.; Mittermaier, A. K.; Rubinstein, J. L.; Kay, L. E. “An Allosteric Switch Regulates Mycobacterium Tuberculosis ClpP1P2 Protease Function as Established by Cryo-EM and Methyl-TROSY NMR”. Proc. Natl. Acad. Sci. U. S. A. 2020, 117 (11), 5895–5906. https://doi.org/10.1073/pnas.1921630117. *Co-first authorship.


      Ripstein, Z. A.; Vahidi, S.; Houry, W. A.; Rubinstein, J. L.; Kay, L. E. “A Processive Rotary Mechanism Couples Substrate Unfolding and Proteolysis in the ClpXP Degradation Machinery”. Elife. 2020, 9, e52158. https://doi.org/10.7554/eLife.52158.

2019

      Rubinstein, J. L.; Guo, H.; Ripstein, Z. A.; Haydaroglu, A.; Au, A.; Yip, C. M.; Trani, J. M. D.; Benlekbira, S.; Kwoka, T. “Shake-It-off: A Simple Ultrasonic Cryo-EM Specimen-Preparation Device” Acta Crystallogr. Sect. D Struct. Biol. 2019, 75 (12), 1063–1070. https://doi.org/10.1107/S2059798319014372.


      Huang, R.; Ripstein, Z. A.; Rubinstein, J. L.; Kay, L. E. “Cooperative Subunit Dynamics Modulate P97 Function”. Proc. Natl. Acad. Sci. U. S. A. 2019, 116 (1), 158–167. https://doi.org/10.1073/pnas.1815495116.

2018

      Vahidi, S.*; Ripstein, Z. A.*; Bonomi, M.; Yuwen, T.; Mabanglo, M. F.; Juravsky, J. B.; Rizzolo, K.; Velyvis, A.; Houry, W. A.; Vendruscolo, M.; Rubinstein, J. L.; Kay, L. E. “Reversible Inhibition of the ClpP Protease via an N-Terminal Conformational Switch”. Proc. Natl. Acad. Sci. U. S. A. 2018, 115 (28), E6447–E6456. https://doi.org/10.1073/pnas.1805125115. *Co-first authorship.

2017

      Ripstein, Z. A.; Huang, R.; Augustyniak, R.; Kay, L. E.; Rubinstein, J. L. “Structure of a AAA+ Unfoldase in the Process of Unfolding Substrate”. Elife. 2017, 6, e25754. https://doi.org/10.7554/eLife.25754.

2016

       Ripstein, Z. A.; Rubinstein, J. L. “Processing of Cryo-EM Movie Data”. In Methods in Enzymology; Crowther, R. A., Ed.; Methods in Enzymology; Academic Press, 2016; Vol. 579, pp 103–124. https://doi.org/10.1016/bs.mie.2016.04.009.


       Huang, R.*; Ripstein, Z. A.*; Augustyniak, R.; Lazniewski, M.; Ginalski, K.; Kay, L. E.; Rubinstein, J. L. “Unfolding the Mechanism of the AAA+ Unfoldase VAT by a Combined Cryo-EM, Solution NMR Study”. Proc. Natl. Acad. Sci. U. S. A. 2016, 113 (29), E4090-W4199. https://doi.org/10.1073/pnas.1603980113. *Co-first authorship.

*Co-first author